● 腫瘤微環境多維度分析 ?
To understand the complex tissue microenvironment under both physiological and pathological conditions such as cancer, it is very important to profile immune checkpoint proteins and phenotypic markers. Spatial localization, co-localization, and proximity of multiple biomarkers are critical when cataloging subsets of tumor infiltrating lymphocytes and cancer cells as well as their interactions in the tumor microenvironment.
Based on T cell-?based? classification? of ?tumours, cancers are defined as four classification: hot immune tumours, altered-?immunosuppressed immune ?tumours, ?altered-?excluded immune ?tumours, cold immune tumours. It is becoming critical to understand the mechanisms responsible for hot, altered or cold immune tumours in order to boost a weak antitumour immunity. Both current and potential therapeutic strategies to best target these four categories of tumour, which in most cases involve combinatorial immunotherapy strategies.
From: Nature reviews | Drug Discovery
Multidimensional Analysis of Tumor Microenvironment at Yicon
Fig.1 IHC staining of human lymphocytes (hCD45 positive) in PBMC humanized xenograft tumors ( A. PBMCCDX xenografts. B. PBMCPDX xenografts.) at Yicon.
Fig.2 Expression of Immune Suppression Genes in TME of PBMC Humanized Models.
Fig.3 A. Immune checkpoint receptor phenotypes in TME. B. Digital quantification of multiple IHC in TME.
Fig.4 IHC staining of the predictive biomarker in patient samples.